Both exponential-decay and square-wave pulses have been used very effectively for both electroporation and electrofusion. Number of bids and bid amounts may be slightly out of date. Detailed parameters can be found for each of these cell lines, with details for the preparation of electrocompetent cells in the Gene Pulser Xcell system instructions or at www. Please check your Internet connection and reload this page.
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Detailed parameters can be found for each of these cell lines, with details for the preparation of electrocompetent cells in the Gene Pulser Xcell system instructions or at www. Choice of Waveforms Choice of Waveforms A choice of exponential or square waveforms.
Both exponential-decay and square-wave pulses have been used very effectively for both electroporation and electrofusion. The shape of the electroporation wave can have a significant effect on the transformation efficiency for different cell types.
The Gene Pulser MXcell system generates both exponential and square waveforms, enabling you to choose the waveform and protocol that will work best for your cells.
Exponential-decay pulse. The value CR is known as the time constant of the voltage decay. The shorter the time constant the faster the decay. Square-wave pulse. Truncating the pulse from a capacitor after discharging it into the sample generates a square-wave pulse. The pulse length is the time the cells are subjected to the discharge. During the pulse, the voltage again decreases by an exponential decay so that at the end of the pulse the voltage is lower than at the beginning. This drop in voltage is called the pulse droop and it is measured as a percentage of the initial voltage.
Transfection Protocol Library Transfection Protocol Library The Transfection Protocol Online Library contains protocols obtained from the literature, developed by Bio-Rad scientists, or submitted by scientists like you. Bio-Rad Laboratories has helped scientists deliver molecules into cells for over 35 years.
These transfection instruments are cited extensively in the literature.
GENE PULSER XCELL PDF
Gene Pulser Xcell Electroporation Systems
Gene Pulser Xcell™ Total System #1652660